解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::A resonance energy transfer assay of membrane fusion developed by P. S. Uster and D. W. Deamer (Biochemistry 24, 1-8 (1985)) was used in a study of influenza haemagglutinin-mediated fusion. The characteristics of fusion and haemolysis by X-31 (H3N2) virus, a number of mutants of X-31 which fuse membranes at higher pH,...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90083-8
更新日期:1986-02-01 00:00:00
abstract::Six mutants of lambda having reduced dependence on the htpR function of Escherichia coli were isolated from lambda cIts857. Burst sizes in htpRts cells at 40.5 degrees were in the range of 10 to 20 particles per cell. Mapping and complementation analysis of one of the mutants suggested that the mutation in this isolat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90093-0
更新日期:1986-02-01 00:00:00
abstract::The nucleotide sequences of the long terminal repeat (LTR) of simian retroviruses (STLV), which are closely related to human T-cell leukemia virus type I (HTLV-I) were found to be of at least two subgroups: an Asian subtype in macaques and an African subtype in African green monkeys and chimpanzee. The nucleotide sequ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90336-3
更新日期:1986-01-30 00:00:00
abstract::We have examined the synthesis of the T protein of the human polyomavirus, JCV, during productive infection in primary cultures of human fetal glial and kidney cells. Immunoprecipitation of protein extracts from virus infected cells revealed that the JCV large T protein from both the prototype Mad and HEK adapted stra...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90418-6
更新日期:1986-01-15 00:00:00
abstract::We described a simple method to introduce site-specific mutations into region E3 of adenovirus (Ad). Mutations are made in cloned Ad2 EcoRI-D (map position 76-83), then ligated between Ad5 EcoRI-A (map position 0-76) and EcoRI-B (map position 83-100) to complete the viral genome. We have used this method to isolate a ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90412-5
更新日期:1986-01-15 00:00:00
abstract::The sequence of the 1491 nucleotides found at the 3' end of the genome of the highly aphid-transmissible (HAT) isolate of tobacco etch virus (TEV) has been determined. The nucleotide sequence of the capsid protein gene has been identified and compared with the corresponding region of the not-aphid-transmissible (NAT) ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90133-3
更新日期:1985-12-01 00:00:00
abstract::During abortive infection of Go/G1-arrested primary baby mouse kidney (BMK) cell cultures with simian virus 40 (SV40), expression of the viral large T antigen is followed by a mitotic host response including the stimulation of host macromolecular synthesis and induction into the cell cycle of Go/G1-arrested cells. We ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90130-8
更新日期:1985-12-01 00:00:00
abstract::The expression of type I murine interferon (MuIFN) genes and several other cellular genes was examined in poly rI.rC induced and Newcastle disease virus (NDV) infected mouse cells. Northern analysis of RNA from induced L cells revealed that the MuIFN-alpha s are expressed efficiently in NDV infected cells but only at ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90140-0
更新日期:1985-12-01 00:00:00
abstract::The transcriptive complex, the nucleocapsid with the viral RNA-synthesizing activity, of Newcastle disease virus (NDV) contains three protein components, the major structural subunit (NP) and two associated proteins (P and L) involved in the RNA synthesis. We studied the pathway of these proteins from synthesis to ass...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90132-1
更新日期:1985-12-01 00:00:00
abstract::The nucleotide sequence of the env gene of a previously described human provirus (ERV3) has been determined beginning near the 3'-end of the pol gene and continuing through the 3'-LTR. Analysis of the nucleotide sequence revealed the presence of a long open reading frame of 1944 nucleotides that is capable of encoding...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90147-3
更新日期:1985-12-01 00:00:00
abstract::Sensitivity of a library of rat cells transformed in vitro with viable recombinant adenoviruses to natural killer (NK) cells and allogeneic cytotoxic T cells has been studied and correlated with their oncogenic potential in syngeneic rats. All cell lines transformed with the sub370-12E1AB virus (containing E1A and E1B...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90143-6
更新日期:1985-12-01 00:00:00
abstract::We have examined the host range of AIDS-associated retroviruses (ARV) that are known to infect human T cells of the helper subset. We have observed that the virus cannot infect fibroblast and epithelial cell lines of many different animal species. It is infectious and replicates efficiently in peripheral mononuclear c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90146-1
更新日期:1985-12-01 00:00:00
abstract::Mouse hepatitis viruses (MHV) of different virulence for mice were studied with respect to interferon (IFN) sensitivity. The growth of low-virulent MHV-S and intermediately virulent MHV-JHM was significantly suppressed in IFN-treated L cells compared with untreated cells. However, a comparable suppression of the growt...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90225-9
更新日期:1985-11-01 00:00:00
abstract::Cytoplasmic poly(A)-containing RNA from parainfluenza virus 3-infected cells was used as template to construct a cDNA library that was cloned into the EcoRV site of the plasmid pMG5. The resulting clones were screened with [32P]-labeled cDNA probes made from infected and mock-infected cell mRNAs. The virus specificity...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90237-5
更新日期:1985-11-01 00:00:00
abstract::A collection of TK+, ACV-resistant mutants of herpes simplex virus type 1 (HSV-1) has been derived using a selection system based on biochemically transformed cells. Evidence is presented suggesting that most of these mutants induce resistant DNA polymerase activities and are thus likely to express variant DNA polymer...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90009-1
更新日期:1985-10-30 00:00:00
abstract::Murine leukemia virus (MuLV) genome encodes a protease (Y. Yoshinaka, I. Katoh, T.D. Copeland, and S. Oroszlan (1985), Proc. Natl. Acad. Sci. USA 82, 1618-1622), which has been shown to cause maturation, specified as morphological conversion from "immature" to "mature" form of virus cores. To examine whether "immature...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90161-8
更新日期:1985-09-01 00:00:00
abstract::At least three human cytomegalovirus polypeptides are targets for virus neutralizing antibody; a single protein of 86,000 molecular weight (p86) and two coimmunoprecipitating proteins of 130,000 and 55,000 molecular weight (p130/55). These polypeptides have been isolated by immunoaffinity chromatography and tested for...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90215-6
更新日期:1985-08-01 00:00:00
abstract::Cell lines persistently infected with foot-and-mouth disease virus (FMDV) have been established by growth of BHK-21 (c-13) or IBRS-2 (c-26) that survived standard cytolytic infections with FMDV. They maintain cytoplasmic FMDV RNA sequences, as shown by dot blot hybridization tests, using cloned FMDV cDNA as probes. Ce...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90198-9
更新日期:1985-08-01 00:00:00
abstract::Following mechanical inoculation of leaves with turnip yellow mosaic virus (TYMV), a significant proportion of the retained inoculum is uncoated within 45 sec, and the process is more or less complete after 2 min. At least 80-90% of the uncoating takes place in the epidermis. The application of virus to the intact lea...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90274-0
更新日期:1985-07-30 00:00:00
abstract::In vitro packaging experiments with phage P22 using artificially ligated plasmid concatemers have shown that the pac site is not necessary for DNA packaging although in vivo this initiation signal is indispensable. This indicates that the phage-coded protein gp3 also executes other important functions during phage mat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90273-9
更新日期:1985-07-30 00:00:00
abstract::Antigenic sites on the G1 glycoprotein of La Crosse bunyavirus were defined by constructing a panel of neutralizing and nonneutralizing monoclonal antibodies (F. Gonzalez-Scarano, R. E. Shope, C. H. Calisher, and N. Nathanson (1982), Virology 120, 42-53). To analyze the relationship between the individual epitopes del...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90283-1
更新日期:1985-07-30 00:00:00
abstract::Lassa virus RNA isolated from purified virus particles was polyadenylated and reverse transcripts were cloned into the PstI site of plasmid pUC9. Clones containing sequences of the smaller (S) segment of the Lassa virus genome were identified by hybridization with purified S RNA. They were characterized by their abili...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90278-8
更新日期:1985-07-30 00:00:00
abstract::Lymphoid cell lines were derived from three avian leukosis virus (ALV)-induced lymphomas. These cell lines contained proviral DNA sequences integrated upstream from the c-myc proto-oncogene, expressed increased levels of c-myc RNA, and were tumorigenic in syngeneic animals. While cell surface immunoglobulin (IgM) was ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90312-5
更新日期:1985-07-15 00:00:00
abstract::A new type of retrovirus (Sm-MTV) released by cultured cells of a spontaneous mammary tumor from a house musk shrew, Suncus murinus, is described. The Sm-MTV is distinct morphologically from type C particles. In spite of certain morphological similarities to type B and type D retroviruses, the Sm-MTV is readily distin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90324-1
更新日期:1985-07-15 00:00:00
abstract::The splice acceptor site of pX gene of HTLV-I has been determined to be at base position 7301 using S1 nuclease protection analysis. This splice acceptor site is used in all HTLV-I immortalized T-cell clones studied despite variation in the abundance levels of pX mRNA. Our results confirmed the proposal by Haseltine e...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90404-0
更新日期:1985-06-01 00:00:00
abstract::Three cDNA clones that express viral gene products in Escherichia coli JM83 were derived from a watermelon mosaic virus-1 strain of papaya ringspot virus (PRSV-W). DNAs complementary to portions of the viral RNA were inserted into the pUC8 and pUC9 plasmids, and the expressed polypeptides were fusion products with the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90383-6
更新日期:1985-06-01 00:00:00
abstract::Sixteen monoclonal antibodies directed to the hemagglutinin-neuraminidase (HN) protein of a 1957 isolate of parainfluenza type 3 virus (PIV3) were produced and used to examine antigenic variation in clinical strains. Analysis of hemagglutination-inhibition reactivity patterns of antigenic variants selected in vitro in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90395-2
更新日期:1985-06-01 00:00:00
abstract::Putative replication complexes of cauliflower mosaic virus (CaMV) which resemble hepatitis B virus replication complexes in several respects have been identified. The CaMV replication complexes contain a heterogeneous population of minus-strand DNA ranging in size from less than 600 nucleotides to full genome length o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90109-6
更新日期:1985-05-01 00:00:00
abstract::The effects of inhibitors, which interfere with oligosaccharide trimming by blocking mannosidases, on the processing and export of the viral glycoproteins of Rous sarcoma virus (RSV), have been studied. 1-Deoxymannojirimycin (DIM) prevents removal of mannose residues from the Man9 (GlcNAc)2 oligosaccharide whereas swa...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90122-9
更新日期:1985-05-01 00:00:00
abstract::A series of BglII- and Sau3A-derived fragments of phage T1 DNA were cloned into the plasmid vector pLV59 and the clones were tested for their ability to complement and recombine with infecting genomes of am mutants representing all known T1 essential genes. The resulting data were used to correlate the T1 genetic and ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90106-0
更新日期:1985-05-01 00:00:00
abstract::In 1943 Burnet reported on changes in the hemagglutinating properties of human influenza virus which occurred during adaptation of the virus to growth in chicken eggs. Only recently has direct evidence been presented that these changes affect the antigenic properties of the virus. Schild et al. (G. C. Schild, J. S. Ox...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90105-9
更新日期:1985-05-01 00:00:00
abstract::The DNA sequence of the gag and pol regions of a provirus cloned from a bovine tumor is presented. In order to confirm these results the sequence of portions of a second clone, derived from a virus-producing cell line, was also determined. The gag gene was found to consist of 1179 nucleotides, which probably encode on...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90344-7
更新日期:1985-04-30 00:00:00
abstract::The 24,000-molecular-weight major internal protein (p24) and the 15,000-molecular-weight nucleic acid binding protein (p15) of human T-cell leukemia virus type II (HTLV-II) were subjected to amino acid composition and amino-terminal amino acid sequence analysis. A comparison of amino acid composition of p24 and p15 of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90434-9
更新日期:1985-04-15 00:00:00
abstract::Lymphocytic choriomeningitis virus (LCMV) Armstrong caused disordered growth and glucose metabolism secondary to growth hormone deficiency in infected C3H/St mice. In contrast, LCMV strain WE replicated in fewer growth hormone-producing cells, failed to disrupt growth hormone synthesis, and did not cause growth hormon...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90431-3
更新日期:1985-04-15 00:00:00
abstract::Two temperature-sensitive mutants of cowpea mosaic virus have been isolated following nitrous acid mutagenesis. One mutant, 8-14, was shown to have a temperature-sensitive defect in the B component RNA. The location of the temperature-sensitive defect of the other mutant, 8-10, was not unambiguously determined. No vir...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90258-2
更新日期:1985-03-01 00:00:00
abstract::A full-size cloned cDNA copy of the rotavirus gene encoding the structural neutralization glycoprotein (VP7) of Nebraska calf diarrhea virus (NCDV), a strain recently shown to be effective as a vaccine in children, has been sequenced. Comparison of the deduced amino acid sequence of NCDV (serotype 6) VP7 with that of ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90260-0
更新日期:1985-03-01 00:00:00
abstract::Visna is a slow virus infection of sheep in which the characteristic pathological change is demyelination in foci of inflammation. The latter is thought to be the result of an immunopathological process directed against cellular and antigenic targets that have been difficult to define because of restricted viral gene ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90264-8
更新日期:1985-03-01 00:00:00
abstract::A dodecapeptide containing the sequence of the C terminus of the nonstructural polyprotein of Sindbis virus has been synthesized and used to immunize rabbits. The antisera obtained precipitated polypeptides from cells infected with the HR strains of Sindbis or with temperature-sensitive mutants ts11 or ts18. Four diff...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90254-5
更新日期:1985-03-01 00:00:00
abstract::The protein p10 that forms the connector of phage phi 29, has been produced in Escherichia coli harboring a plasmid that carried the gene coding for this protein. The connector protein is assembled in a 13.4-S oligomer that has an apparent molecular weight of 460,000, suggesting that it is a dodecamer. The purified ol...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90251-x
更新日期:1985-03-01 00:00:00
abstract::Long-term persistent infections were established with the flavivirus, West Nile virus (WNV), strain E101, in embryofibroblast cultures derived from susceptible C3H/HE and congenic-resistant C3H/RV mice. Cultures were initially maintained by weekly subculture at 37 degrees, but at passage 6 sister cultures were shifted...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90454-4
更新日期:1985-01-15 00:00:00